Appendix A to Part 40 - DOT Standards for Urine Collection
The Collection Kit Contents:
1. Collection Container
a. Single-use container, made of plastic, large enough to easily
catch and hold at least 55 mL of urine voided from the body.
b. Must have graduated volume markings clearly noting levels of
45 mL and above.
c. Must have a temperature strip providing graduated temperature
readings 32-38 º C / 90-100 º F, that is affixed or
can be affixed at a proper level on the outside of the collection
container. Other methodologies (e.g., temperature device built
into the wall of the container) are acceptable provided the temperature
measurement is accurate and such that there is no potential for
contamination of the specimen.
d. Must be individually wrapped in a sealed plastic bag or shrink
wrapping; or must have a peelable, sealed lid or other easily
visible tamper-evident system.
e. May be made available separately at collection sites to address
shy bladder situations when several voids may be required to complete
the testing process.
2. Plastic Specimen Bottles
a. Each bottle must be large enough to hold at least 35 mL; or
alternatively, they may be two distinct sizes of specimen bottles
provided that the bottle designed to hold the primary specimen
holds at least 35 mL of urine and the bottle designed to hold
the split specimen holds at least 20 mL.
b. Must have screw-on or snap-on caps that prevent seepage of
the urine from the bottles during shipment.
c. Must have markings clearly indicating the appropriate levels
(30 mL for the primary specimen and 15 mL for the split) of urine
that must be poured into the bottles.
d. Must be designed so that the required tamper-evident bottle
seals made available on the CCF fit with no damage to the seal
when the employee initials it nor with the chance that the seal
overlap would conceal printed information.
e. Must be wrapped (with caps) together in a sealed plastic bag
or shrink wrapping separate from the collection container; or
must be wrapped (with cap) individually in sealed plastic bags
or shrink wrapping; or must have peelable, sealed lid or other
easily visible tamper-evident system.
f. Plastic material must be leach resistant.
3. Leak-resistant Plastic Bag
a. Must have two sealable compartments or pouches which are leak-resistant;
one large enough to hold two specimen bottles and the other large
enough to hold the CCF paperwork.
b. The sealing methodology must be such that once the compartments
are sealed, any tampering or attempts to open either compartment
will be evident.
4. Absorbent material
Each kit must contain enough absorbent material to absorb the
entire contents of both specimen bottles. Absorbent material must
be designed to fit inside the leak-resistant plastic bag pouch
into which the specimen bottles are placed.
5. Shipping Container
a. Must be designed to adequately protect the specimen bottles
from shipment damage in the transport of specimens from the collection
site to the laboratory (e.g., standard courier box, small cardboard
box, plastic container).
b. May be made available separately at collection sites rather
than being part of an actual kit sent to collection sites.
c. A shipping container is not necessary if a laboratory courier
hand-delivers the specimen bottles in the plastic leak-proof bags
from the collection site to the laboratory.
Appendix B to Part 40 - DOT Drug Testing Semi-Annual
The following items are required on each report:
Reporting Period: (inclusive dates)
Laboratory Identification: (name and address)
Employer Identification: (name; may include billing code or ID
C/C/TPA Identification: (where applicable; name and address)
1. Number of specimen results reported: (total number)
By test type:
(a) Pre-employment testing: (number)
(b) Post-accident testing: (number)
(c) Random testing: (number)
(d) Reasonable suspicion/cause testing: (number)
(e) Return-to-duty testing: (number)
(f) Follow-up testing: (number)
(g) Type not noted on CCF: (number)
2. Number of specimens reported as (a) Negative: (total number)
(b) Negative-dilute: (number)
3. Number of specimens reported as Rejected for Testing: (total
(a) Fatal flaw: (number)
(b) Uncorrected flaw: (number)
4. Number of specimens reported as Positive: (total number)
(a) Marijuana Metabolite: (number)
(b) Cocaine Metabolite: (number)
(1) Codeine: (number)
(2) Morphine: (number)
(3) 6-AM: (number)
(d) Phencyclidine: (number)
(e) Amphetamines: (number)
(1) Amphetamine: (number)
(2) Methamphetamine: (number):
5. Adulterated: (number)
6. Substituted: (number)
7. Invalid results: (number)
Appendix C to Part 40 – [Reserved]
Appendix D to Part 40 - Report Format: Split Specimen
Failure to Reconfirm
Fax or mail to:
Department of Transportation
Office of Drug and Alcohol Policy and Compliance
400 7th Street, SW, Room 10403
Washington, DC 20590
(fax) 202 366-3897
1. MRO name, address, phone number, and fax number.
2. Collection site name, address, and phone number.
3. Date of collection.
4. Specimen I.D. number.
5. Laboratory accession number.
6. Primary specimen laboratory name, address, and phone number.
7. Date result reported or certified by primary laboratory.
8. Split specimen laboratory name, address, and phone number.
9. Date split specimen result reported or certified by split
10. Primary specimen results (e.g., name of drug, adulterant)
in the primary specimen.
11. Reason for split specimen failure-to-reconfirm result (e.g.,
drug or adulterant not present, specimen invalid, split not collected,
12. Actions taken by the MRO (e.g., notified employer of failure
to reconfirm and requirement for recollection).
13. Additional information explaining the reason for cancellation.
14. Name of individual submitting the report (if not the MRO).
Appendix E to Part 40 - SAP Equivalency Requirements for
1. Experience: Minimum requirements are for three years of full-time
supervised experience or 6,000 hours of supervised experience
as an alcoholism and/or drug abuse counselor. The supervision
must be provided by a licensed or certified practitioner. Supervised
experience is important if the individual is to be considered
a professional in the field of alcohol and drug abuse evaluation
2. Education: There exists a requirement of 270 contact hours
of education and training in alcoholism and/or drug abuse or related
training. These hours can take the form of formal education, in-service
training, and professional development courses. Part of any professional
counselor's development is participation in formal and non-formal
education opportunities within the field.
3. Continuing Education: The certified counselor must receive
at least 40 - 60 hours of continuing education units (CEU) during
each two year period. These CEUs are important to the counselor's
keeping abreast of changes and improvements in the field.
4. Testing: A passing score on a national test is a requirement.
The test must accurately measure the application of the knowledge,
skills, and abilities possessed by the counselor. The test establishes
a national standard that must be met to practice.
5. Testing Validity: The certification examination must be reviewed
by an independent authority for validity (examination reliability
and relationship to the knowledge, skills, and abilities required
by the counseling field). The reliability of the exam is paramount
if counselor attributes are to be accurately measured. The examination
passing score point must be placed at an appropriate minimal level
score as gauged by statistically reliable methodology.
6. Measurable Knowledge Base: The certification process must
be based upon measurable knowledge possessed by the applicant
and verified through collateral data and testing. That level of
knowledge must be of sufficient quantity to ensure a high quality
of SAP evaluation and referral services.
7. Measurable Skills Base: The certification process must be
based upon measurable skills possessed by the applicant and verified
through collateral data and testing. That level of skills must
be of sufficient quality to ensure a high quality of SAP evaluation
and referral services.
8. Quality Assurance Plan: The certification agency must ensure
that a means exists to determine that applicant records are verified
as being true by the certification staff. This is an important
check to ensure that true information is being accepted by the
9. Code of Ethics: Certified counselors must pledge to adhere
to an ethical standard for practice. It must be understood that
code violations could result in de-certification. These standards
are vital in maintaining the integrity of practitioners. High
ethical standards are required to ensure quality of client care
and confidentiality of client information as well as to guard
against inappropriate referral practices.
10. Re-certification Program: Certification is not just a one-time
event. It is a continuing privilege with continuing requirements.
Among these are continuing education, continuing state certification,
and concomitant adherence to the code of ethics. Re-certification
serves as a protector of client interests by removing poor performers
from the certified practice.
11. Fifty State Coverage: Certification must be available to
qualified counselors in all 50 states and, therefore, the test
must be available to qualified applicants in all 50 states. Because
many companies are multi-state operators, consistency in SAP evaluation
quality and opportunities is paramount. The test need not be given
in all 50 states but should be accessible to candidates from all
12. National Commission for Certifying Agencies (NCCA) Accreditation:
Having NCCA accreditation is a means of demonstrating to the Department
of Transportation that your certification has been reviewed by
a panel of impartial experts that have determined that your examination(s)
has met stringent and appropriate testing standards.
Appendix F to Part 40 - Drug and Alcohol Testing Information
that C/TPAs May Transmit to Employers
1. If you are a C/TPA, you may, acting as an intermediary, transmit
the information in the following sections of this part to the
DER for an employer, if the employer chooses to have you do so.
These are the only items that you are permitted to transmit to
the employer as an intermediary. The use of C/TPA intermediaries
is prohibited in all other cases, such as transmission of laboratory
drug test results to MROs, the transmission of medical information
from MROs to employers, the transmission of SAP reports to employers,
the transmission of positive alcohol test results, and the transmission
of medical information from MROs to employers.
2. In every case, you must ensure that, in transmitting the information,
you meet all requirements (e.g., concerning confidentiality and
timing) that would apply if the party originating the information
(e.g., an MRO or collector) sent the information directly to the
employer. For example, if you transmit MROs' drug testing results
to DERs, you must transmit each drug test result to the DER in
compliance with the requirements for MROs set forth in §40.167.
Drug testing information
§40.25: Previous two years’ test results
§40.35: Notice to collectors of contact information for DER
§40.61(a): Notification to DER that an employee is a "no
show" for a drug test
§40.63(e): Notification to DER of a collection under direct
§40.65(b)(6) and (7) and (c)(2) and (3): Notification to
DER of a refusal to
provide a specimen or an insufficient specimen
§40.73(a)(9): Transmission of CCF copies to DER (However,
MRO copy of CCF
must be sent by collector directly to the MRO, not through the
§40.111(a): Transmission of laboratory statistical report
§40.127(f): Report of test results to DER
§40.127(g), 40.129(d), 40.159(a)(4)(ii); 40.161(b): Reports
to DER that test is cancelled
§40.129 (d) Report of test results to DER
§40.129(g)(1): Report to DER of confirmed positive test in
§§40.149(b): Report to DER of changed test result
§40.155(a): Report to DER of dilute specimen
§40.167(b) and (c): Reports of test results to DER
§40.187(a) – (f) Reports to DER concerning the reconfirmation
§40.191(d): Notice to DER concerning refusals to test
§40.193(b)(3): Notification to DER of refusal in shy bladder
§40.193(b)(4): Notification to DER of insufficient specimen
§40.193(b)(5): Transmission of CCF copies to DER (not to
§40.199: Report to DER of cancelled test and direction to
DER for additional collection
§40.201: Report to DER of cancelled test
Alcohol testing information
§40.215: Notice to BATs and STTs of contact information
§40.241(b)(1): Notification to DER that an employee is a
"no show" for an alcohol test
§40.247(a)(2): Transmission of alcohol screening test results
only when the test result is less than 0.02
§40.255(a)(4): Transmission of alcohol confirmation test
results only when the test result is less than 0.02
§40.263(a)(3) and 263(b)(3): Notification of insufficient
saliva and failure to provide sufficient amount of breath
Appendix G to Part 40 – Alcohol Testing Form
The following form is the alcohol testing form required for use
in the DOT alcohol testing program beginning August 1, 2001. Use
of the form is authorized beginning January 18, 2001.